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PC-3M人前列腺癌细胞鉴定报告

阅读次数:1175  发布时间:2019/5/5 9:01:18
  

 

 

 

 

 

 

STR Profiling Report

 

 

 

 

 

Sample From: EK-Bioscience

Sample Type: Cell Line

Testing Method: STR Genotyping

Report Time: 12/20.2018

 

 


 

Cell Line Authentication – STR Profiling Report

 

Sample code

Table 1. Sample Code

Customer’s code

Company Code

PC-3M

20181210-01

 

Sample Number:1

Sample Type: Cell line                             

Testing Type: STR

Testing Method:

DNA was extracted by a commercial kit from CORNING (AP-EMN-BL-GDNA-250G). The twenty STRs including Amelogenin locus were amplified by six multiplex PCR and separated on ABI 3730XL Genetic Analyzer. The signals were then analyzed by the software GeneMapper.

Data Interpretation:

Cell lines were authenticated using Short Tandem Repeat (STR) analysis asdescribed in 2012 in ANSI Standard (ASN-0002) by the ATCC Standards Development Organization (SDO) and in Capes-Davis et al., Match criteria for human cell line authentication: Where do we draw the line? Int J Cancer.2013;132(11):2510-9.

 

 

 

 

 

 

 

 

 

 

 

 

 


Test Results:

1. STR profile

Table 2. STR and Amelogenin Genotyping Results of Cell line 20181210-01.

Loci

Sample information

Cell Bank information

Sample name:PC-3M

Cell line name:PC-3M

Allele1

Allele2

Allele3

Allele1

Allele2

Allele3

D5S818

13

13

 

13

13

 

D13S317

11

11

 

11

11

 

D7S820

8

11

 

8

11

 

D16S539

11

11

 

 

 

 

VWA

17

17

 

17

17

 

TH01

6

7

 

6

7

 

AMEL

X

X

 

X

X

 

TPOX

8

9

 

8

9

 

CSF1PO

11

11

 

11

11

 

D12S391

21

21

 

 

 

 

FGA

24

24

 

24

24

 

D2S1338

18

20

 

 

 

 

D21S11

29

31.2

 

 

 

 

D18S51

14

15

 

 

 

 

D8S1179

13

13

 

 

 

 

D3S1358

16

16

 

16

16

 

D6S1043

18

18

 

 

 

 

PENTAE

10

17

 

 

 

 

D19S433

14

14

 

 

 

 

PENTAD

9

9

 

 

 

 

D1S1656

12

16

 

 

 

 

 

2. database annotation

Figure 1. STR matching analysis

                                                

Note: The STR online match analysis of the test cell against EXPASY database, showing cell number (Cell No.CVCL_9555) and cell name.

 

3. Authentication

The submitted profile is similar to the following DSMZ human cell line: /.

Note: A cell line can be considered to be authenticated when 80% (exact match) of the alleles in its STR profile match profiles from tissue or other cell line samples from that donor or from database. Cell lines with between a 55% to 80% (similar) match require further profiling for investigation of relatedness.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Appendix:

 

1. Genotyping Strategy and Site Distribution

Table S1. Experimental Strategy and Sites

 

Strategy 1

Strategy 2

Strategy 3

Strategy 4

1

D3S1358

D8S1179

D19S433

AMEL

2

VWA

D21S11

TH01

D1S1656

3

D7S820

D16S539

D13S317

D5S818

4

CSF1PO

D2S1338

TPOX

D12S391

5

PENTAE

PENTAD

D18S51

FGA

6

 

 

D6S1043

 

The allele match algorithm compares the 8 core loci plus amelogenin only, even though alleles from all lociwill be reported when available.

2. DSMZ tools was used to carry on the cell line comparison, which contains 2455 cell lines STR data from ATCC, DSMZ, JCRB ,ECACCGNE and RIKEN databases. If the cell is not included in the above cell library, users need to compared with other databases.

 

Technician: Jianan Zhang

Checked by:  Ning Qian

Issued by: Yang Bai

Issue date:5/5/2019

 

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