| 英文名称DACT1 中文名称肝癌新基因3蛋白抗体 别 名Dact1 / Dapper homolog 1; Dact; Dact1; DACT1_HUMAN; Dapper antagonist of beta catenin homolog 1; Dapper antagonist of catenin 1; Dapper antibodyDapper homolog 1; DAPPER1; Dpr antibodyDpr1; Frd; Frodo; hDPR1; Hepatocellular carcinoma novel gene 3 protein; Heptacellular carcinoma novel gene 3; HNG3; MTNG3; Thyex3. 研究领域 抗体来源Rabbit 克隆类型Polyclonal 交叉反应Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, 产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分 子 量90kDa 细胞定位细胞核 细胞浆 细胞外基质 性 状Liquid 浓 度1mg/ml 免 疫 原KLH conjugated synthetic peptide derived from human DACT1:751-836/836 亚 型IgG 纯化方法affinity purified by Protein A 储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 ａnd 50% Glycerol. 保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMedPubMed 产品介绍The protein encoded by this gene belongs to the dapper family, characterized by the presence of PDZ-binding motif at the C-terminus. It interacts with, ａnd positively regulates dishevelled-mediated signaling pathways during development. Depletion of this mRNA from xenopus embryos resulted in loss of notochord ａnd head structures, ａnd mice lacking this gene died shortly after birth from severe posterior malformations. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] Function: Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome-dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB. Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 ａnd DVL2; the function seems to be independent of canonical Wnt signaling ａnd rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway (By similarity). The nuclear form may prevent the formation of LEF1:CTNNB1 complex ａnd recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling. May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, ａnd dendrite formation ａnd establishment of spines. Subunit: Can form homodimers ａnd heterodimers with DACT2 or DACT3. Interacts with CSNK1D, PKA catalytic subunit, PKC-type kinase, CSNK2A1, CSNK2B, DVL1, DVL3, VANGL1, VANGL2, CTNND1 ａnd HDAC1 (By similarity). Interacts with DVL2. Interacts with YWHAB; the interaction is enhanced by PKA phosphorylating DACT1 at Ser-237 ａnd Ser-827. Interacts with CTNNB1 ａnd HDAC1. Interacts with GSK3B; the interaction is indicative for an association of DACT1 with the beta-catenin destruction complex. Interacts with GSK3A. Subcellular Location: Cytoplasm. Nucleus. Cell junction, synapse. Note=Shuttles between the nucleus ａnd the cytoplasm. Seems to be nuclear in the absence of Wnt signaling ａnd to translocate to the cytoplasm in its presence. Post-translational modifications: DISEASE: Defects in DACT1 may be a cause of susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are congenital malformations of the central nervous system ａnd adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele ａnd spina bifida, which result from the failure of fusion in the cranial ａnd spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic ａnd environmental components. Similarity: Belongs to the dapper family. SWISS: Q9NYF0 Gene ID: 51339 Database links: Entrez Gene: 51339 Human Entrez Gene: 59036 Mouse Entrez Gene: 500666 Rat Entrez Gene: 723789 Chicken Omim: 607861 Human SwissProt: Q9NYF0 Human SwissProt: Q8R4A3 Mouse Unigene: 48950 Human Unigene: 714204 Human Unigene: 46662 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. | 产品图片 | Tissue/cell: human HepG2 cells;4% Paraformaldehyde-fixed ａnd paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DACT1 Polyclonal Antibody, Unconjugated(bs-10304R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. | |
